PRODUCT CODE: EM1701-80

AMACR/P504S Monoclonal Antibody (EM1701-80)

  • IVD–IHC

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of AMACR/P504S on Siha cell and human liver tissue lysates using anti-AMACR/P504S antibody at 1/1,000 dilution.
  • Western blot analysis of AMACR/P504S on Siha cell and human liver tissue lysates using anti-AMACR/P504S antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with AMACR/P504S antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis of AMACR/P504S on Siha cell and human liver tissue lysates using anti-AMACR/P504S antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

AMACR/P504S Monoclonal Antibody (EM1701-80)

Immunogen

Recombinant full length protein of human amacr.

Host

Mouse

Positive Control

Siha cell and human liver tissue lysates, Human liver tissue, human colon cancer tissue, human prostate cancer tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

6F5

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

42 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

AMACR/P504S

SYNONYMS

2 arylpropionyl CoA epimerase antibody; 2 methylacyl CoA racemase antibody; 2-methylacyl-CoA racemase antibody; Alpha methylacyl CoA racemase antibody; Alpha methylacyl Coenzyme A racemase antibody; Alpha methylacyl-CoA racemase deficiency, included antibody; Alpha-methylacyl-CoA racemase antibody; Amacr antibody; AMACR deficiency, included antibody; AMACR_HUMAN antibody CBAS4 antibody; Da1-8 antibody; EC 5.1.99.4 antibody; Macr1 antibody; Methylacyl CoA racemase alpha antibody; RACE antibody; RM antibody

SEQUENCE SIMILARITIES

Belongs to the CoA-transferase III family.

SUBCELLULAR LOCATION

Mitochondrion. Peroxisome.

FUNCTION

AMACR, is an enzyme that responsible for converting (2R)-methylacyl-CoA esters to their (2S)-methylacyl-CoA epimers and known substrates, including coenzyme A esters of pristanic acid (mostly derived from phytanic acid, a 3-methyl branched-chain fatty acid that is abundant in the diet) and bile acids derived from cholesterol. Both decreased and increased levels of the enzyme in humans are linked with diseases. Reduction of the protein level or activity results in the accumulation of (2R)-methyl fatty acids such as bile acids which causes neurological symptoms. Increased levels of AMACR protein concentration and activity are associated with prostate cancer, and the enzyme is used widely as a biomarker (known in cancer literature as P504S) in biopsy tissues. Increased levels of AMACR are also associated with some breast, colon, and other cancers, but it is unclear exactly what the role of AMACR is in these cancers. This AMACR antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalinfixed, paraffin-embedded tissue sections using IHC test methods.