PRODUCT CODE: em1701-80

AMACR/P504S Monoclonal Antibody (EM1701-80)

  • IVD–IHC

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of AMACR/P504S on Siha cell and human liver tissue lysates using anti-AMACR/P504S antibody at 1/1,000 dilution.
  • Western blot analysis of AMACR/P504S on Siha cell and human liver tissue lysates using anti-AMACR/P504S antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with AMACR/P504S antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis of AMACR/P504S on Siha cell and human liver tissue lysates using anti-AMACR/P504S antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

AMACR/P504S Monoclonal Antibody (EM1701-80)

Immunogen

Recombinant full length protein of human amacr.

Host

Mouse

Positive Control

Siha cell and human liver tissue lysates, Human liver tissue, human colon cancer tissue, human prostate cancer tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

6F5

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

42 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Alpha-methylacyl-CoA racemase

GENE NAME

AMACR

SYNONYMS

AMACR

SEQUENCE SIMILARITIES

Belongs to the CoA-transferase III family.

SUBCELLULAR LOCATION

Peroxisome. Mitochondrion.

FUNCTION

Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters. Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs.