PRODUCT CODE: ER1706-60

AMACR Antibody (ER1706-60)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of AMACR on different lysates using anti-AMACR antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse liver tissue <br />
 Lane 2: SiHa<br />
 Lane 3: SW480<br />
 Lane 4: Human kidney tissue<br />
  • Western blot analysis of AMACR on different lysates using anti-AMACR antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse liver tissue <br />
 Lane 2: SiHa<br />
 Lane 3: SW480<br />
 Lane 4: Human kidney tissue<br />
  • ICC staining AMACR in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AMACR antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-AMACR antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AMACR antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of PC-3M cells with AMACR antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of AMACR on different lysates using anti-AMACR antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse liver tissue
Lane 2: SiHa
Lane 3: SW480
Lane 4: Human kidney tissue

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

AMACR Antibody (ER1706-60)

Immunogen

Recombinant protein.

Host

Rabbit

Positive Control

Human kidney tissue, mouse liver tissue lysate, Siha, SW480, HepG2, human liver tissue, human colon cancer tissue, PC-3M.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

42 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Alpha-methylacyl-CoA racemase

GENE NAME

AMACR

SYNONYMS

AMACR

SEQUENCE SIMILARITIES

Belongs to the CoA-transferase III family.

SUBCELLULAR LOCATION

Peroxisome. Mitochondrion.

FUNCTION

Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters. Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs.