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PRODUCT CODE: em1701-75

ALDH4A1 Monoclonal Antibody (EM1701-75)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

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Western blot analysis of ALDH4A1 on SK-Br-3 (1) and HepG2 (2) cell lysate using anti-ALDH4A1 antibody at 1/1,000 dilution.
  • Western blot analysis of ALDH4A1 on SK-Br-3 (1) and HepG2 (2) cell lysate using anti-ALDH4A1 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-ALDH4A1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ALDH4A1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ALDH4A1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ALDH4A1 antibody. Counter stained with hematoxylin.
Western blot analysis of ALDH4A1 on SK-Br-3 (1) and HepG2 (2) cell lysate using anti-ALDH4A1 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

ALDH4A1 Monoclonal Antibody (EM1701-75)

Immunogen

Synthetic peptide of c terminal human aldh4a1.

Host

Mouse

Positive Control

SK-Br-3, HepG2, human liver tissue, human colon cancer tissue, human prostate tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

7000

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

62 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

P30038

PROTEIN NAME

Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial

GENE NAME

ALDH4A1

SYNONYMS

P5C dehydrogenase, ALDH4A1, ALDH4, P5CDH

SEQUENCE SIMILARITIES

Belongs to the aldehyde dehydrogenase family.

TISSUE SPECIFICITY

Highest expression is found in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas.

SUBCELLULAR LOCATION

Mitochondrion matrix.

FUNCTION

Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.