PRODUCT CODE: EM1701-21

AKR1C1 Monoclonal Antibody (EM1701-21)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of AKR1C1 on different lysates using anti-AKR1C1 antibody at 1/2,000 dilution.<br />
  Positive control:<br />
  Lane 1: HepG2<br />
  Lane 2: Human liver tissue
  • Western blot analysis of AKR1C1 on different lysates using anti-AKR1C1 antibody at 1/2,000 dilution.<br />
  Positive control:<br />
  Lane 1: HepG2<br />
  Lane 2: Human liver tissue
  • ICC staining AKR1C1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with AKR1C1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti mouse IgG was used as the secondary antibody.
Western blot analysis of AKR1C1 on different lysates using anti-AKR1C1 antibody at 1/2,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Human liver tissue

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

AKR1C1 Monoclonal Antibody (EM1701-21)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

HepG2, human liver tissue, MCF-7, human pancreas tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

5-F10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

37 kDa

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:2,000-1:10,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

AKR1C1

SYNONYMS

2-dihydrobenzene-1 antibody; 2-diol dehydrogenase antibody; 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody; 20 ALPHA HSD antibody; 20 alpha hydroxysteroid dehydrogenase antibody; 20-alpha-HSD antibody; 20-alpha-hydroxysteroid dehydrogenase antibody; 20ALPHAHSD antibody; 2ALPHAHSD antibody; AK1C1 antibody; AK1C1_HUMAN antibody; AKR1C1 antibody; Aldo keto reductase family 1 member C1 antibody; aldo-keto reductase C antibody; Aldo-keto reductase family 1 member C1 antibody; Aldo-keto reductase family, 1 member 1 antibody; C9 antibody; Chlordecone reductase homolog antibody; Chlordecone reductase homolog HAKRC antibody; DD1 antibody; DD1/DD2 antibody; DDH antibody; DDH1 antibody; Dihydrodiol dehydrogenase 1 antibody; Dihydrodiol dehydrogenase 1/2 antibody; dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase antibody; dihydrodiol dehydrogenase isoform DD1 antibody; Dihydrodiol dehydrogenase, type 1 antibody; H37 antibody; HAKRC antibody; HBAB antibody; Hepatic dihydrodiol dehydrogenase antibody; High affinity hepatic bile acid-binding protein antibody; High-affinity hepatic bile acid-binding protein antibody; Indanol dehydrogenase antibody; MBAB antibody; MGC8954 antibody; Trans-1 antibody; Trans-1,2 dihydrobenzene 1,2 diol dehydrogenase antibody; Type II 3 alpha hydroxysteroid dehydrogenase antibody

SEQUENCE SIMILARITIES

Belongs to the aldo/keto reductase family.

TISSUE SPECIFICITY

Expressed in all tissues tested including liver, prostate, testis, adrenal gland, brain, uterus, mammary gland and keratinocytes. Highest levels found in liver, mammary gland and brain.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Human liver contains isoforms of dihydrodiol dehydrogenase (DD1, DD2, DD3 and DD4), which belong to the aldo-oxo reductase / aldo-keto reductase (AKR) superfamily, have 20α- or 3α-hydroxysteroid dehydrogenase AKD1C3 and 3α-HSD are alternate designations for human DD3 (which is referred to as AKR1C18 in rodents), while DD4 is also designated as AKR1C1, DDH or DDH1, while DD2 also can be designated AKR1C2, dDD, BABP or DDH2. AKR1C4, CD, CHDR or AKR1C6 (in rodents). DD1 and DD2 are 20α-HSDs, DD3 and DD4 are the 3α-HSDs. The 20α-HSD catalyzes the reaction of progesterone to the inactive form 20α-hydroxyprogesterone. The 3α-HSD is a cytosolic, monomeric, NADPH-depen DD1 and DD2 are ubiquitously expressed, whereas DD4 mRNA is restricted to the liver. DD3 is a unique enzyme that can specifically catalyze the dehydrogenation of trans-benzene dihydrodiol and trans- naphthalenedihydrodiol.