Positive control:
Lane 1: A549 cell lysate
Lane 2: Siha cell lysate
Applications
-
WB
-
ICC
-
IHC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
AKR1B1 Antibody (ER2001-28)
Immunogen
Synthetic peptide within human akr1b1 aa 200-260.
Host
Rabbit
Positive Control
A549 cell lysate, Siha cell lysate, Siha, human seminal vesicle tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
36 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
ICC
-
1:50-1:200
-
IHC
-
1:100-1:500
TARGET
UNIPROT #
PROTEIN NAME
AKR1B1
SYNONYMS
ADR antibody; AKR1B 1 antibody; Akr1b1 antibody; Aldehyde reductase 1 antibody; Aldehyde reductase antibody; Aldo keto reductase family 1, member B1 antibody; Aldo-keto reductase family 1 member B1 antibody; aldo-keto reductase family 1, member B1 (aldose reductase) antibody; Aldose reductase antibody; aldr 1 antibody; ALDR_HUMAN antibody; aldr1 antibody; ALR2 antibody; AR antibody; Lii5 2 CTCL tumor antigen antibody; Low Km aldose reductase antibody; MGC1804 antibody
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia . Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal . Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides. Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadi