PRODUCT CODE: ER80601

AFP Antibody (ER80601)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of AFP on different cell lysates using anti-AFP antibody at 1/1000 dilution.<br />
 Positive control: <br />
 Lane 1: HepG2 <br />
 Lane 2: human lung <br />
 Lane 3: human liver
  • Western blot analysis of AFP on different cell lysates using anti-AFP antibody at 1/1000 dilution.<br />
 Positive control: <br />
 Lane 1: HepG2 <br />
 Lane 2: human lung <br />
 Lane 3: human liver
  • ICC staining AFP in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining AFP in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with AFP antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of AFP on different cell lysates using anti-AFP antibody at 1/1000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: human lung
Lane 3: human liver

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

AFP Antibody (ER80601)

Immunogen

Peptide

Host

Rabbit

Positive Control

NCCIT, HepG2, Jurkat,human liver tissue, human lung tissue, human liver cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

AFP

SYNONYMS

Afp antibody; AFPD antibody; Alpha fetoglobulin antibody; Alpha fetoprotein antibody; Alpha fetoprotein precursor antibody; Alpha-1-fetoprotein antibody; Alpha-fetoglobulin antibody; Alpha-fetoprotein antibody; alpha-fetoprotein, Hereditary persistence of, included antibody; FETA antibody; FETA_HUMAN antibody; Hereditary persistence of alpha fetoprotein antibody; HPAFP antibody

SEQUENCE SIMILARITIES

Belongs to the ALB/AFP/VDB family.

TISSUE SPECIFICITY

Plasma. Synthesized by the fetal liver and yolk sac.

DEVELOPMENTAL STAGE

Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.

POST-TRANSLATIONAL MODIFICATION

Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.; Sulfated.

SUBCELLULAR LOCATION

Secreted

FUNCTION

AFP is a protein normally produced by the fetal liver and is present in the fluid surrounding the fetus (amniotic fluid), and crosses the placenta into the mother's blood. AFP gene expression is regulated by the interactions between steroid hormone receptors and transcriptional factors in separate signal transduction pathways. AFP functions as a binding and transporting ligand and cell growth regulator. Elevated expression level of AFP has been implicated in colorectal, ovarian, pancreatic, testicular, and certain liver cancers. High levels of AFP are also seen in some diseases such as hepatitis and colitis.