Positive control:
Lane 1: HepG2
Lane 2: human lung
Lane 3: human liver
Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
AFP Antibody (ER80601)
Immunogen
Peptide
Host
Rabbit
Positive Control
NCCIT, HepG2, Jurkat,human liver tissue, human lung tissue, human liver cancer tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified
MOLECULAR WEIGHT
70 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC
-
1:200
-
IHC-P
-
1:200
-
FC
-
1:100-1:200
TARGET
UNIPROT #
PROTEIN NAME
AFP
SYNONYMS
Afp antibody; AFPD antibody; Alpha fetoglobulin antibody; Alpha fetoprotein antibody; Alpha fetoprotein precursor antibody; Alpha-1-fetoprotein antibody; Alpha-fetoglobulin antibody; Alpha-fetoprotein antibody; alpha-fetoprotein, Hereditary persistence of, included antibody; FETA antibody; FETA_HUMAN antibody; Hereditary persistence of alpha fetoprotein antibody; HPAFP antibody
SEQUENCE SIMILARITIES
Belongs to the ALB/AFP/VDB family.
TISSUE SPECIFICITY
Plasma. Synthesized by the fetal liver and yolk sac.
DEVELOPMENTAL STAGE
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
POST-TRANSLATIONAL MODIFICATION
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.; Sulfated.
SUBCELLULAR LOCATION
Secreted
FUNCTION
AFP is a protein normally produced by the fetal liver and is present in the fluid surrounding the fetus (amniotic fluid), and crosses the placenta into the mother's blood. AFP gene expression is regulated by the interactions between steroid hormone receptors and transcriptional factors in separate signal transduction pathways. AFP functions as a binding and transporting ligand and cell growth regulator. Elevated expression level of AFP has been implicated in colorectal, ovarian, pancreatic, testicular, and certain liver cancers. High levels of AFP are also seen in some diseases such as hepatitis and colitis.