PRODUCT CODE: R1308-12

Active+Pro Caspase-9 Antibody (R1308-12)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis on Hela cell lysates using anti-Caspase-9 rabbit polyclonal antibodies with Dilution at 1:500 (1) and 1:1000 (2).
  • Western blot analysis on Hela cell lysates using anti-Caspase-9 rabbit polyclonal antibodies with Dilution at 1:500 (1) and 1:1000 (2).
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Caspase-9 rabbit polyclonal antibody.
Western blot analysis on Hela cell lysates using anti-Caspase-9 rabbit polyclonal antibodies with Dilution at 1:500 (1) and 1:1000 (2).

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Active+Pro Caspase-9 Antibody (R1308-12)

Immunogen

Synthetic peptide within human caspase-9 aa 280-313.

Host

Rabbit

Positive Control

Hela, human lung carcinoma.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

46 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000- 1:2,000

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Caspase-9

GENE NAME

CASP9

SYNONYMS

CASP-9,APAF-3,ICE-LAP6

SEQUENCE SIMILARITIES

Belongs to the peptidase C14A family.

TISSUE SPECIFICITY

Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

DEVELOPMENTAL STAGE

Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.

POST-TRANSLATIONAL MODIFICATION

Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.; Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.

FUNCTION

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).; Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.