PRODUCT CODE: R1512-19

Myosin light-chain 2 Antibody (R1512-19)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Myosin Light Chain 2 on different lysates using anti-Myosin Light Chain 2 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse skeletal muscle<br />
 Lane 2: Human skeletal muscle<br />
 Lane 3: Mouse heart<br />
 Lane 4: Rat heart
  • Western blot analysis of Myosin Light Chain 2 on different lysates using anti-Myosin Light Chain 2 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse skeletal muscle<br />
 Lane 2: Human skeletal muscle<br />
 Lane 3: Mouse heart<br />
 Lane 4: Rat heart
  • ICC staining Myosin Light Chain 2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Myosin Light Chain 2 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Myosin Light Chain 2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Myosin Light Chain 2 on different lysates using anti-Myosin Light Chain 2 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse skeletal muscle
Lane 2: Human skeletal muscle
Lane 3: Mouse heart
Lane 4: Rat heart

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Myosin light-chain 2 Antibody (R1512-19)

Immunogen

Peptide

Host

Rabbit

Positive Control

Mouse skeletal muscle tissue lysate, human skeletal muscle tissue lysate, mouse heart tissue, rat heart tissue, A549, Hela, C2C12.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

19 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Myosin Light Chain 2

SYNONYMS

Cardiac myosin light chain-2 antibody; Cardiac ventricular myosin light chain 2 antibody; CMH10 antibody; MLC 2v antibody; MLC-2 antibody; MLC-2v antibody; MLC2 antibody; MLRV_HUMAN antibody; MYL 2 antibody; MYL2 antibody; Myosin light chain 2 regulatory cardiac slow antibody; Myosin light polypeptide 2 regulatory cardiac slow antibody; Myosin regulatory light chain 2 antibody; Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody; Regulatory light chain of myosin antibody; RLC of myosin antibody; Slow cardiac myosin regulatory light chain 2 antibody; ventricular/cardiac muscle isoform antibody

POST-TRANSLATIONAL MODIFICATION

N-terminus is methylated by METTL11A/NTM1.; Phosphorylated by MYLK3 and MYLK2; promotes cardiac muscle contraction and function (By similarity). Dephosphorylated by PPP1CB complexed to PPP1R12B (By similarity). The phosphorylated form in adult is expressed as gradients across the heart from endocardium (low phosphorylation) to epicardium (high phosphorylation); regulates cardiac torsion and workload distribution (By similarity).

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Encoded by the MYL2 gene, myosin regulatory light chain 2, ventricular/cardiac muscle isoform, also designated MLC-2 or MLC2v, is part of a hexameric complex of two heavy chains and four light chains predominantly expressed in adult cardiac ventricle muscle. Myosin regulatory light chain 2 binds calcium and has been shown to be a useful molecular marker for cardiac chamber specification. The co-expression of myosin regulatory light chain 7 (MYL7) and myosin regulatory light chain 2 in the outflow tract and atrioventricular canal, together with the single expression in the atrial (MYL7) and ventricular (MYL2) myocardium, permits the delineation of their boundaries. At the amino acid level there is 96% homology between the human and mouse myosin regulatory light chain sequences. Mutations in MYL2 are correlated with mid-left ventricular chamber type hypertrophic cardiomyopathy (MVC2) as well as familial hypertrophic cardiomyopathy type 10 (CMH10).