Applications
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WB
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
6X His tag (C terminal) Monoclonal Antibody (M0812-3)
Immunogen
Synthetic peptide (klh-coupled)
Host
Mouse
Positive Control
C-terminal His-tagged recombinant protein, N-terminal His-tagged recombinant protein.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
8-F0
PROPERTIES
Form
Liquid
Storage Condition
-20C.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol.
Concentration
2 ug/ul
PURIFICATION
Protein G affinity purified.
Isotype
IgG2a
APPLICATION DILUTION
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WB
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1:2,000
TARGET
PROTEIN NAME
6X His tag (C terminal)
SYNONYMS
6 His epitope tag antibody; Hexa His tag antibody; HHHHHH epitope tag antibody; HHHHHH tag antibody; His tag antibody; Polyhistidine Tag antibody
FUNCTION
A variety of plasmids contain DNA that encodes a tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. This antibody can be used to detect the carboxyl-terminal 6×his tag in recombinant protein.
CITATIONS
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You, Qiang et al.
Interaction of AIM with insulin-like growth factor-binding protein-4. | International Journal of Molecular Medicine [2015]
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WB
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CoIP
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human
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Citation
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Wang, Boshi et al.
POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation. | Nature Communications [2015]
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WB
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human
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Citation
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Liao, Xiangzhi et al.
Prokaryotic expression, purification and characterization of human cyclooxygenase-2. | International Journal of Molecular Medicine [2017]
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WB
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human
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Citation
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Sheng, Xiangpeng et al.
Bacterial effector NleL promotes enterohemorrhagic E. coli-induced attaching and effacing lesions by ubiquitylating and inactivating JNK. | PloS Pathogens [2017]
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CoIP
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IF
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human
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Citation
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Wang, Meng et al.
High glucose-induced ubiquitination of G6PD leads to the injury of podocytes. | Faseb Journal : Official Publication of the Federation of American Societies for Experimental Biology [2019]
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WB
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human
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Citation
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