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PRODUCT CODE: M0812-3

6X His tag (C terminal) Monoclonal Antibody (M0812-3)

Applications

  • WB

REACTIVITY

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Western blot analysis on recombinant protein with His tag using anti-6X His tag antibody (M0812-3).
  • Western blot analysis on recombinant protein with His tag using anti-6X His tag antibody (M0812-3).
Western blot analysis on recombinant protein with His tag using anti-6X His tag antibody (M0812-3).

Applications

  • WB

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

6X His tag (C terminal) Monoclonal Antibody (M0812-3)

Immunogen

Synthetic peptide (klh-coupled)

Host

Mouse

Positive Control

C-terminal His-tagged recombinant protein, N-terminal His-tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

8-F0

PROPERTIES

Form

Liquid

Storage Condition

-20C.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol.

Concentration

2 ug/ul

PURIFICATION

Protein G affinity purified.

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:2,000

TARGET

PROTEIN NAME

6X His tag (C terminal)

SYNONYMS

6 His epitope tag antibody; Hexa His tag antibody; HHHHHH epitope tag antibody; HHHHHH tag antibody; His tag antibody; Polyhistidine Tag antibody

FUNCTION

A variety of plasmids contain DNA that encodes a tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. This antibody can be used to detect the carboxyl-terminal 6×his tag in recombinant protein.

CITATIONS

  • You, Qiang et al.

    Interaction of AIM with insulin-like growth factor-binding protein-4. | International Journal of Molecular Medicine [2015]

  • Wang, Boshi et al.

    POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation. | Nature Communications [2015]

  • Liao, Xiangzhi et al.

    Prokaryotic expression, purification and characterization of human cyclooxygenase-2. | International Journal of Molecular Medicine [2017]

  • Sheng, Xiangpeng et al.

    Bacterial effector NleL promotes enterohemorrhagic E. coli-induced attaching and effacing lesions by ubiquitylating and inactivating JNK. | PloS Pathogens [2017]

  • Wang, Meng et al.

    High glucose-induced ubiquitination of G6PD leads to the injury of podocytes. | Faseb Journal : Official Publication of the Federation of American Societies for Experimental Biology [2019]