PRODUCT CODE: ER1706-64

4E-BP1 Antibody (ER1706-64)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of 4E-BP1 on different lysates using anti-4E-BP1 antibody at 1/500 dilution.<br />
  Lane 1: PC-3M <br />
  Lane 2: Mouse placenta tissue
  • Western blot analysis of 4E-BP1 on different lysates using anti-4E-BP1 antibody at 1/500 dilution.<br />
  Lane 1: PC-3M <br />
  Lane 2: Mouse placenta tissue
  • ICC staining 4E-BP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-4E-BP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-4E-BP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-4E-BP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-4E-BP1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of PC-3M cells with 4E-BP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of 4E-BP1 on different lysates using anti-4E-BP1 antibody at 1/500 dilution.
Lane 1: PC-3M
Lane 2: Mouse placenta tissue

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

4E-BP1 Antibody (ER1706-64)

Immunogen

Recombinant protein.

Host

Rabbit

Positive Control

Mouse placenta tissue, PC-3M, 293T, rat esophagus tissue, human tonsil tissue, human pancreas tissue, mouse small intestine tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

12.5 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

4E-BP1

SYNONYMS

4E-BP1 antibody; 4EBP1 antibody; 4EBP1_HUMAN antibody; BP 1 antibody; eIF4E binding protein 1 antibody; eIF4E-binding protein 1 antibody; Eif4ebp1 antibody; Eukaryotic translation initiation factor 4E-binding protein 1 antibody; PHAS-I antibody; PHASI antibody; Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody

SEQUENCE SIMILARITIES

Belongs to the eIF4E-binding protein family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E.; Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E.