PRODUCT CODE: ER1803-44

43924 Antibody (ER1803-44)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of APR3 on human skin tissue lysates using anti-APR3 antibody.<br />
 Lane 1: Anti-APR3 antibody (1/500).<br />
 Lane 2: Anti-APR3 antibody, pre-incubated with the immunizaiton peptide.<br />
  • Western blot analysis of APR3 on human skin tissue lysates using anti-APR3 antibody.<br />
 Lane 1: Anti-APR3 antibody (1/500).<br />
 Lane 2: Anti-APR3 antibody, pre-incubated with the immunizaiton peptide.<br />
  • ICC staining APR3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining APR3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-APR3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with APR3 antibody at 1/100 dilution (Pink purple) compared with an unlabelled control (cells without incubation with primary antibody; Yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of APR3 on human skin tissue lysates using anti-APR3 antibody.
Lane 1: Anti-APR3 antibody (1/500).
Lane 2: Anti-APR3 antibody, pre-incubated with the immunizaiton peptide.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

43924 Antibody (ER1803-44)

Immunogen

Synthetic peptide corresponding to mouse kif3a c terminal.

Host

Rabbit

Positive Control

Human skin tissue lysates, A549, SH-SY-5Y, mouse liver tissue, MCF-7.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

25 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

All-trans retinoic acid-induced differentiation factor

GENE NAME

ATRAID

SYNONYMS

APR-3, ATRAID, APR3, C2orf28, HSPC013, UNQ214/PRO240

TISSUE SPECIFICITY

Weakly expressed in hematopoietic cell lines.

SUBCELLULAR LOCATION

Nucleus envelope. Cell membrane; Single-pass membrane protein. Note=Colocalizes with NELL1 on the nuclear envelope and the perinuclear region.

FUNCTION

Promotes osteoblast cell differentiation and terminal mineralization. Plays a role in inducing the cell cycle arrest via inhibiting CCND1 expression in all-trans-retinoic acid (ATRA) signal pathway.