PRODUCT CODE: 0407-5

14-3-3 beta/alpha Antibody (0407-5)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis on different cell and tissue using anti-14-3-3 b/a polyclonal antibody.
  • Western blot analysis on different cell and tissue using anti-14-3-3 b/a polyclonal antibody.
  • ICC staining 14-3-3 b/a in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis on different cell and tissue using anti-14-3-3 b/a polyclonal antibody.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

14-3-3 beta/alpha Antibody (0407-5)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, human brain, mouse brain, N2A

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

28 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:5,000

  • ICC

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

14-3-3 protein beta/alpha

GENE NAME

Ywhab

SYNONYMS

KCIP-1

SEQUENCE SIMILARITIES

Belongs to the 14-3-3 family.

POST-TRANSLATIONAL MODIFICATION

Isoform alpha differs from isoform beta in being phosphorylated (By similarity). Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.; Isoform Short contains a N-acetylmethionine at position 1.

SUBCELLULAR LOCATION

Cytoplasm. Melanosome.

FUNCTION

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.