Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T cell proliferation, cytokine production, differentiation of effector T cells, and the induction of Bcl-x, a promoter of T cell survival. Conversely, engagement of CTLA4 by B7-1 or B7-2 may inhibit proliferation and IL-2 production. Pdcd-1L1 (programmed cell death ligand-1), also known as B7-H1 or PD-L1, is 290 amino acid type I transmembrane protein which is 20% and 15% identical to B7-1 and B7-2, respectively. Pdcd-1L2 has immunoglobulin V-like and C-like domains and a 30 amino acid cytoplasmic tail. It does not bind CD28, cytotoxic T-lymphocyte A4 or ICOS (inducible co-stimulator). IL-2, although produced in small amounts, is required for the effect of Pdcd-1L1 co-stimulation. The gene which encodes Pdcd-1L1 maps to human chromosome 9p24. Pdcd-1L2 (programmed cell death ligand-2) is a 73 amino acid protein which contains a signal sequence, IgV- and IgC-like domains, a transmembrane region and a cytoplasmic region. The gene which encodes Pdcd-1L2 maps to human chromosome 9p24.2. The constitutive expression of Pdcd-1L1 and Pdcd-1L2 on paren-chymal cells of heart, lung and kidney suggests that the Pdcd-1-Pdcd-L system could provide unique negative signaling to help prevent autoimmune disease.
Human, Mouse, Rat
Recombinant Rabbit Monoclonal Antibody
Human tonsil tissue, mouse heart tissue, human melanoma tissue, human lung cancer tissue.
B7 H antibody
B7 H1 antibody
B7 homolog 1 antibody
CD 274 antibody
CD274 antigen antibody
CD274 molecule antibody
PD L1 antibody
PDCD1 ligand 1 antibody
PDL 1 antibody
Programmed cell death 1 ligand 1 antibody
Programmed death ligand 1 antibody
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
ProA affinity purified
Cell membrane, Endomembrane system.
Fig1: Western blot analysis of PD-L1 on mouse heart lysates using anti-PD-L1 antibody at 1/1,000 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD-L1 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-PD-L1 antibody. Counter stained with hematoxylin.
1. Jurado J.O., et al. 2008. Programmed death (PD)-1:PD-ligand 1/PD-ligand 2 pathway inhibits T cell effector functions during human tuberculosis. J. Immunol. 181:116-125.
2. Boorjian S.A., et al. 2008. T-cell coregulatory molecule expression in urothelial cell carcinoma: clinicopathologic correlations and association with survival. Clin. Cancer Res. 14:4800-4808.
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