Ribulose bisphosphate carboxylase/oxygenase activase is an enzyme localized to chloroplasts which activates Rubisco by promoting ATP-dependent conformational changes. Alternative name: RuBisCo activase RCA. There are two forms of activase (alpha and beta) in some species (for example Arabidopsis, camelina, spinach, rice) and only one form in other species (tobacco, maize,Chlamydomonas). Alpha is about 46-47 kDa, beta is about 42 kDa. Species that have only one form have the beta form.
A. thaliana, N. tabacum, O. sativa, Z.mays, S.oleracea, M. alba
WB, IHC, IF
Rabbit Polyclonal Antibody
Mulberry leaf, rice, A. thaliana, tabacum
3 phospho D glycerate carboxylase antibody
Ribulose 1,5 bisphosphate carboxylase/oxygenase small subunit N methyltransferase I antibody
Ribulose bisphosphate carboxylase large chain antibody
RuBisCO large subunit antibody
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Peptide affinity purified
Fig1: Western blot analysis on different tissue lysates using anti-Rubisco activase rabbit polyclonal antibody.
Lane 1: Mulberry leaf
Lane 2: Rice
Lane 3: A. thaliana
Lane 4: Tabacum
Fig2： Immunohistochemical analysis of paraffin-embedded A. thaliana using anti-Rubisco activase rabbit polyclonal antibody.
Fig3：Immunofluorescence staining of paraffin- embedded A. thaliana using anti-Rubisco activase rabbit polyclonal antibody.
1. Jurczyk et al. Evidence for alternative splicing mechanisms in meadow fescue (Festuca pratensis) and perennial ryegrass (Lolium perenne) Rubisco activase gene. J Plant Physiol 176:61-64 (2015).
2. Yin et al. Characterization of Rubisco activase genes in maize: an α-isoform gene functions alongside a β-isoform gene. Plant Physiol 164(4):2096-106 (2014).
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To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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